FIG 3.

WW domain 1 of WWOX interacts with AMOT. Extracts from HEK293T cells transfected with myc-tagged WT or the indicated mutants of WWOX and HA-tagged AMOT were subjected to IP. WWOX and AMOT in the precipitates (A) and cell extracts (B) were subjected to Western blotting. * indicates the IgG heavy chain. (C) Quantification of the AMOT protein levels in panel B from three independent experiments. Statistical significance was analyzed by a one-way ANOVA. **, P < 0.01; ****, P < 0.0001. (D) Schematic diagram of WWOX with key domains highlighted. Y33, W44, and P47 are three crucial amino acids in WW1 domain that mediate binding to the PY motif. NLS, nuclear localization signal; SDR, short-chain dehydrogenase/reductase domain.