FIG 8.

WWOX induces lysosomal degradation of AMOT. (A) HEK293T cells were transfected with the indicated combinations of plasmids, including lysosome marker mCherry-LAMP1 (red), HA-tagged AMOT (green), and myc-tagged WWOX (white). Cells were visualized using confocal microscopy. Scale bars, 10 μm. The enlarged view and arrows highlight colocalization of AMOT and LAMP1 in lysosomes. (B) HEK293T cells were transfected with YFP-AMOT and mCherry-LAMP1 plus vector alone (Control) or WWOX. Live cells were observed via spinning disk confocal microscopy, beginning at 12 h posttransfection. Representative images showing the localization of AMOTp130 in control and WWOX-expressing cells at each hour during observation. Scale bars, 10 μm.