TABLE 1.

Commercially available HTLV diagnostic immunoassays

Assay type	Assay (manufacturer)a	Principle of assay
Enzyme immunoassay	Elecsys HTLV-I/II (Roche Diagnostics)	One-step double-antigen sandwich chemiluminescent immunoassay (ECLIA); detects antibodies to viral recombinant antigens gp21 and p24.
Enzyme immunoassay	Abbott Architect rHTLV-I/II (Abbott Laboratories)	Two-step chemiluminescent immunoassay (CLIA); detects antibodies to viral synthetic peptide gp46 and recombinant antigen gp21.
Enzyme immunoassay	Ortho Avioq HTLV-I/II Microelisa (Avioq, Inc.)*	Detects antibodies to purified viral lysate and recombinant HTLV-1 p21E antigen.
Enzyme immunoassay	Abbott Prism HTLV-I/HTLV-II (Abbott Laboratories)*	Three-step sandwich chemiluminescent immunoassay (CLIA); detects antibodies to HTLV-1 and HTLV-2 antigens.
Enzyme immunoassay	Murex HTLV I+II (Diasorin)	Detects antibodies to HTLV-1 and HTLV-2 antigens.
Enzyme immunoassay	Gold ELISA HTLV-1/2 (Rem Indústria e Comércio LTDA)	Uses recombinant antigens (gp46 and gp21) fixed on microplate; detects anti-HTLV-1 and HTLV-2 IgM, IgG and IgA antibodies in human serum or plasma by EIA.
Line immunoassay	Inno-LIA HTLV-I/II (Fujirebio, Inc.)	Purified recombinant proteins (rp19, rp24: I/II and rgp21: I/II) and synthetic peptides (gp46: I/II and p19: I) fixed on nylon membrane.
Particle agglutination	Serodia-HTLV-I PA (Fujirebio, Inc.)	Gelatin particle-coated cell culture derived HTLV-1 antigens are agglutinated in presence of antibodies to HTLV-1 in human serum/plasma.
Western blot	HTLV Blot 2.4 (MP Diagnostics)*	Seropositivity criteria for HTLV-1 includes reactivity to p19 Gag, with or without p24 Gag, and to GD21 with the presence of rgp46-I peptide, MTA-1.

^a*, FDA licensed.