Table 1.

Bacterial strains

Strain name	Genotype/description	Source/reference
SL1344 (wild-type, WT)	rpsL hisG	[126]
SL1344_SL1483::cat	Chloramphenicol resistance cassette insertion in the SL1483 pseudogene	[79]
ihfA::cat	Chloramphenicol resistance cassette inserted downstream of the ihfA ORF	This work
ihfB::kan	Kanamycin resistance cassette inserted downstream of the ihfB ORF	This work
2xihfA	2 ihfA copies, ihfB ORF replaced with the ihfA ORF that has the kanamycin resistance cassette inserted downstream – ΔihfB.	This work
2xihfB	2 ihfB copies, ihfA ORF replaced with the ihfB ORF that has the chloramphenicol resistance cassette inserted downstream – ΔihfA	This work
OrfSwap ihfA -ihfB	IhfA and ihfB ORFs reciprocally exchanged	This work
OrfSwap ihfA-ihfB _SL1483::cat	Insertion of a chloramphenicol resistance cassette into the pseudogene SL1483 in the OrfSwap ihfA-ihfB background	This work
SL1344_PprgH::GFP	PprgH-gfp[LVA]/R::cat/ fusion of a gfp gene encoding a destabilized version of GFP to the SPI-1 promoter, PprgH	This work
OrfSwap ihfA-ihfB _PprgH::GFP	Fusion of a gfp derivative encoding a destabilized version of GFP to the SPI-1 promoter, PprgH in the OrfSwap ihfA-ihfB background	This work
SL1344_PssaG::GFP	PprgH-gfp[LVA]/R::cat/ fusion of a gfp derivative encoding a destabilized version of GFP to the SPI-2 promoter, PssaG	This work
OrfSwap ihfA-ihfB _PssaG::GFP	Fusion of a gfp derivative encoding a destabilized version of GFP to the SPI-2 promoter, PssaG in the OrfSwap ihfA-ihfB background	This work