Fig 5. RsbS residue S56 is essential for the transduction of the low pH signal and activation of σB.
Expression of σB-dependent genes (A) lmo2230 and (B) lmo0596, obtained from mid-log phase cultures of wild type and rsbS (S56A) strains untreated (-) and treated (+) for 15 min in pH 5.0 at 37°C. Gene expression is represented as Log2 relative gene expression. (C) Acid challenge of mid-log phase cultures treated in pH 5.0 for 15 min at 37°C, as previously described in Fig 2D. Survival data is expressed as Log10 (CFU.mL-1). Statistical analysis was performed using a paired Student’s t-test relative to the wild type untreated after 15 min, for RT-qPCR data, and untreated wild type at time 0 min, for acid challenge data (*, P value of <0.05; **, P value of <0.01; ***, P value of <0.001).