Skip to main content
. 2022 Mar 11;18(3):e1010103. doi: 10.1371/journal.pgen.1010103

Fig 9. The loss of box C/D snoRNPs reduced mitochondrial health and function.

Fig 9

Measurement of (A) mitochondrial mass, (B) mitochondrial membrane potential, and (C-D) oxygen consumption rate of wild-type (N2) worms reared on E. coli expressing RNAi targeting empty vector (EV) or the box C/D snoRNP machinery (fib-1/FBL, nol-56/Nop56, or nol-58/Nop58). Mitochondrial parameters were measured on basal levels (A-C) or after 8 hours treatment with 25 μM rotenone or DMSO control (D). (E) Mitochondrial membrane potential or (F) oxygen consumption rate was normalized to mitochondrial mass. Three biological replicates with ~400 (mitochondrial mass and membrane potential measurements) or 6,000 (oxygen consumption rate measurements) worms/replicate were analyzed. p values were determined from one-way or two-way ANOVA, followed by Dunnett’s test. *p < 0.05, ** p < 0.01, *** p < 0.001. (D) Purple significance marks indicate comparison between fib-1(RNAi), nol-56(RNAi), or nol-58(RNAi) and EV(RNAi) in DMSO, red significance marks indicate comparison between fib-1(RNAi), nol-56(RNAi), or nol-58(RNAi) and EV(RNAi) in rotenone.