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. 2022 Feb 11;11(3):e220069. doi: 10.1530/EC-22-0069

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Immunofluorescence and -histochemistry staining of asprosin in placental cells and tissue. (A) Immunofluorescent staining of endogenous asprosin in placental BeWo cells with asprosin antibody and negative control (stained solely with Cy5 goat-anti-rabbit secondary antibody). White arrows point to fibrillar asprosin stainings. Scale bar = 20µm. (B) IHC AEC staining of asprosin in different placental areas with respective H&E-G staining and NC. Black arrows display endothelial cells, white arrows show immune cells and the gray arrow points to a decidual cell. Scale bar = 50 µm. H&E, hematoxylin and eosin; IHC, immunohistochemical; NC, negative control.