Figure 1.

Dapagliflozin ameliorated cholesterol-induced cytotoxicity in mice hepatocytes with T2DM. The T2DM mice models were established, and (a) CE and (b) FC tended to be enriched in the liver tissues collected from T2DM mice, which were decreased by Dapagliflozin co-treatment. (c) The expression levels of cell cycle-associated genes in mice liver tissues were screened by performing the Real-Time qPCR analysis. (d) MTT assay and (e) trypan blue staining assay were respectively conducted to examine the effects of Dapagliflozin on cell proliferation and viability in cholesterol-treated hepatocytes. (f) FCM assay was employed to determine the Annexin V-FITC and PI-positive apoptotic cell ratio in mice hepatocytes. We performed each experiment with at least three repetitions, and *P < 0.05 was confirmed as statistical significance.