Figure 2.

Dapagliflozin alleviated cholesterol overloading-induced oxidative damages in mice hepatocytes. The mRNA levels of (a) Nrf2 and (b) HO-1 in mice liver tissues were examined by conducting Real-Time qPCR analysis. (c) The GSH/GSSG ratio in mice liver was examined by using the corresponding commercial kit. (d) DHE staining assay was performed, and DHE-positive cells were counted under immunofluorescent microscope to evaluate ROS generation. (e) MDA levels in mice hepatocytes were evaluated. Real-Time qPCR was performed to determine (f) Nrf2 mRNA and (g) HO-1 mRNA levels in mice hepatocytes. (h) GSH/GSSG ratio in mice hepatocytes was examined. We performed each experiment with at least three repetitions, and *P < 0.05 was confirmed as statistical significance.