Figure 4.

Dapagliflozin re-activated the anti-oxidant Nrf2/HO-1 pathway in cholesterol-treated mice hepatocytes through activating the PI3K/Akt pathway. (a, b) The effects of Dapagliflozin and LY294002 co-treatment on the expression levels of Nrf2 and HO-1 were determined by conducting Western blot analysis. (c) Immunofluorescent staining assay was performed to evaluate the expression levels and cellular localization of Nrf2 in mice hepatocytes. (d-f) Western blot analysis was used to measure the expression status of p-PI3K, PI3K, p-Akt and Akt in the hepatocytes. We performed each experiment with at least three repetitions, and *P < 0.05 was confirmed as statistical significance.