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. 2022 Mar 17;13(1):502–513. doi: 10.1080/21505594.2022.2040188

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — U0126 specifically inhibits IAV-induced NLRP3 inflammasome activation by downregulating pro-IL-1β mRNA. (a–h) H3N2 (MOI = 2) infected THP-1 macrophages for 24 h, and then the cells were stimulated with U0126 (10 μM) or Bay11–7082 (20 μM) for 6 h. the mRNA levels of the indicated genes (a–e) were analyzed by qPCR. Secreted IL-1β protein was detected by ELISA assay (f). Pro-IL-1β and GAPDH proteins were detected by Western blotting (g). Pro-Caspase-1, mature Caspase-1, and GAPDH proteins were detected by Western blotting (h). THP-1 macrophages were treated with DMSO or U0126 (10 μM). the mRNA levels of the indicated genes (i–j) were analyzed by qPCR, while the indicated protein levels were analyzed by Western blotting (k).