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Plasma membrane PI(4)P is important for cell wall integrity. The indicated strains (wild type, PY4861; efr3Δ/Δ, PY4036; efr3Δ/Δ+EFR3, PY4039; ypp1Δ/Δ, PY4033; ypp1Δ/Δ+YPP1, PY4040; stt4Δ/Δ, PY5111; stt4Δ/Δ+STT4, PY5131) were incubated on YEPD with or without caspofungin (125 ng/mL), fluconazole (10 μg/mL), calcofluor white (25 μg/mL), or Congo red (400 μg/mL) for 3 days at 30°C. Similar results were observed in two independent experiments.
