FIG 4.
The stt4 deletion mutant has a thicker cell wall with increased mannan, glucan, and exposed β(1,3)-glucan. (A) Transmission electron micrographs of the indicated strains (wild type, PY4861; stt4Δ/Δ, PY5111) (top), with higher magnification of the cell wall (bottom). Bars, 5 μm (top) and 1 μm (bottom). (B) Quantitation of cell wall thickness from electron micrographs. (C) The stt4 mutant has increased exposure of β(1,3)-glucan together with increased levels of mannan and glucan. Flow cytometry analyses of cells (wild type, PY4861; stt4Δ/Δ, PY5111) labeled with anti-β(1,3)-glucan antibodies and a fluorescently labeled secondary antibody, calcofluor white, fluorescently labeled concanavalin A, and aniline blue. Flow cytometry profiles from one biological replicate (105 gated events; left) and means from three biological replicates normalized to each wild-type mean (right). Error bars indicate standard deviations. * P < 0.05; ****, P < 0.0001; ns, not significant.