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The number of Golgi cisternae is not affected by a decrease in plasma membrane PI(4)P. (A and B) Strains expressing Golgi PI(4)P reporter, FAPP1-GFP (wild type, PY2578; efr3Δ/Δ, PY3933; ypp1Δ/Δ, PY3951; stt4Δ/Δ, PY5552) were imaged, and maximum projections of representative cells are shown with an inverted LUT (left). Quantitation of the number of Golgi cisternae per cell (right) in the indicated strains (24 to 34 cells per strain). Bar, 5 μm.
