Figure 2.
MiR-130b-3p knockdown represses CRC cell proliferation and promotes apoptosis.
(a) RT-qPCR was used to verify the transfection efficiency of miR-130b-3p inhibitor in SW480 and SW620 cells. (b) The viability of SW480 and SW620 cells transfected with NC inhibitor or miR-130b-3p inhibitor was measured using CCK-8 assay. (c-d) The proliferative ability of SW480 and SW620 cells transfected with NC inhibitor or miR-130b-3p inhibitor was assessed using EdU assay. (e) Flow cytometric analysis was performed to assess the apoptosis rate of CRC cells after transfection with NC inhibitor or miR-130b-3p inhibitor. (f) Western blotting analysis was performed to measure the Bax, Bcl-2 and cleaved-caspase-3 protein levels in SW480 and SW620 cells transfected with NC inhibitor or miR-130b-3p inhibitor. (g-h) The number of TUNEL-positive CRC cells after transfection with NC inhibitor or miR-130b-3p inhibitor was evaluated with TUNEL assay. Data are presented as the mean ± SD from three independent experiments. P values were calculated using Student’s t-test. *p < 0.05, **p < 0.01.