Figure 6.

CHD9 is targeted by miR-130b-3p.

(a) RT-qPCR was used to verify the transfection efficiency of miR-130b-3p mimics in SW480 and SW620 cells. (b-c) The CHD9 mRNA and protein levels in SW480 and SW620 cells transfected with NC mimics or miR-130b-3p mimics were measured using RT-qPCR and Western blotting. (d) A Schematic diagram of the potential binding site between CHD9 and miR-130b-3p. (e) SW480 and SW620 cells were transfected with each plasmid, in conjunction with miR-130b-5p mimics or NC mimics, and luciferase activity was measured at 48 h. (f) Expression correlation between miR-130b-3p and CHD9 in COAD samples. (g) Expression correlation between miR-130b-3p and CHD9 in the collected CRC samples. Data are presented as the mean ± SD from three independent experiments. Comparisons between two groups were analyzed using Student’s t-test. Multiple comparisons were analyzed using one-way ANOVA, post-hoc least significant difference (LSD) test. *p < 0.05, **p < 0.01.