Figure 8.

The relationship between circPVT1, miR-124-3p and EZH2.

Notes: The level of EZH2 in LA tissues and adjacent tumor tissues from 60 LA patients was determined by qRT-PCR (A) (n = 60). After transfection, qRT-PCR (B) and Western blot (C) were employed to measure the EZH2 expression in macrophages. The binding of miR-124-3p and EZH2 in the 3ʹUTR region of EZH2 was predicted by TargetScan (D) (n = 3). The binding relationship between miR-124-3p and EZH2 was verified by dual-luciferase reporter assay (E) (n = 3); *P < 0.01, compared to the Normal group; *P < 0.05, ***P < 0.001, compared to the si-NC group, inhibitor NC group or si-circPVT1 group; **P < 0.01, compared to the mimic+mut-EZH2 group; LA, lung adenocarcinoma.