Figure 6.

Intracellular redox status of bloodstream T. b. brucei treated with TryS’s inhibitors. (A) The bloodstream form of a redox reporter cell line of T. brucei was treated for 4 h with the most potent compounds targeting TryS and T. brucei added at concentrations corresponding to 1× or 2× their EC₅₀ (Table 2). The thiol-specific oxidant Diamide (D, 250 µM for 20 min) was included as control. (B) Samples treated with compounds exerting significant changes in biosensor fluorescence, namely 4 and 5 both at 2 × EC₅₀, were treated for 20 min with menadione (M, 250 µM) and DTT (1 mM) to confirm the redox basis of changes observed, respectively. In both plots, the values corresponding to the % reduction of the biosensor are normalised against conditions yielding full biosensor reduction (DTT 1 mM, 20 min) and oxidation (menadione 250 µM for 20 min) in parasites grown in medium containing DMSO 1% v/v.