Table 1.
UHPLC-HRMS parameters for barbiturate analysis.
| Compound | Chemical formula | Retention time (min) | Ionization form | MS1 (m/z) | MS2 (m/z) | |
|---|---|---|---|---|---|---|
| UHPLC condition I | Barbital | C18H12N2O3 | 4.50 | [M-H] | 183.07752 | 140.07207 85.00452 |
| Phenobarbital | C12H12N2O3 | 5.65 | [M-H] | 231.07797 | 188.07214 85.00438 |
|
| Amobarbital/Pentobabital | C11H18N2O3 | 6.37 | [M-H] | 225.12511 | 182.11923 85.00434 |
|
| Secobarbital | C12H18N2O3 | 6.63 | [M-H] | 237.12515 | 194.11917 85.00447 |
|
| Butalbital | C11H16N2O3 | 5.98 | [M-H] | 223.10882 | 180.10294 85.00420 |
|
| Thiopental sodium | C11H17NaN2O3 | 7.12 | [M-Na] | 241.10162 | 57.97557 100.98112 |
|
| Phenobarbita-d5 | C12H17N2O3 | 5.65 | [M-H] | 236.07728 | 193.10257 85.00412 |
|
| UHPLC condition II | Pentobabital | C11H18N2O3 | 11.28 | [M-H] | 225.12511 | 182.11923 85.00434 |
| Amobarbital | C11H18N2O3 | 12.05 | [M-H] | 225.12511 | 182.11923 85.00434 |
|
| Phenobarbita-d5 | C12H17N2O3 | 4.60 | [M-H] | 236.07728 | 193.10257 85.00412 |