Analysis of non-digested genomic DNA (lane 1) and plasmid DNA (lane 2) from E. faecium HM1032 and of nondigested plasmid DNA from the transformant E. faecium HM1070SR (lane 3) by agarose gel electrophoresis and hybridization. (A) Agarose gel electrophoresis of DNA; (B to E) hybridization to ermAM, satA, vanA, and vgb probes, respectively. Linear (chromosomal) DNA is indicated on the left of the gel (Chr). ermAM, satA, and vanA probes hybridized to the same large plasmid; the vgb probe hybridized to the chromosomal DNA of E. faecium HM1032.