Figure 8.
TIMS spectra of a mixture of twelve different ProcA3.3 variants after modification by ProcM and removal of the leader peptide. For comparison of the extracted data with data on individual purified peptides, see Figure S29. Peaks annotated with * correspond to partially cyclized lanthipeptide variants that contain a free Cys and that were alkylated with NEM in the work-flow (see experimental details). ProcA3.3wt/C971H represents a mixture of peptides with overlapping and non-overlapping rings reported previously46 that was used as standard.