Figure 6.

Relative expression of mRNA and proteins involved in inflammation, apoptosis, and autophagy in DBA/2 and D2-mdx hearts at 18 weeks. Expression of (a) IL-6 mRNA and (b) TLR4 mRNA obtained from RT-qPCR and presented as individual ΔCT values, where a lower ΔCT denotes greater mRNA expression. Relative protein expression of (c) Bax, (d) Bcl-2, (e) Bax/Bcl-2 ratio, (f) caspase-3, (g) Beclin-1, and (h) LC3B obtained from western immunoblots. Intact western blots are shown in Supplement 1 Data analysed by Mann–Whitney U test and presented as median and individual values; ^∗p < 0.05 and ^∗∗p < 0.01.