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. 2022 Mar 16;2022:7346460. doi: 10.1155/2022/7346460

Figure 5.

Figure 5

MMP2-AS1 induces MMP2 expression by targeting miR-34c-5p. (a) The CAKI-1 and 786-O cells were transfected with miR-34c-5p mimic or inhibitor. The expression of miR-34c-5p was measured by qPCR. (b, c) The CAKI-1 and 786-O cells were transfected with miR-34c-5p mimic. The luciferase activity of MMP2-AS1 and MMP2 3′UTR was measured by luciferase reporter gene assay. (d) The expression of miR-34c-5p was analyzed by qPCR in CAKI-1 and 786-O cells treated with MMP2-AS1 siRNA. (e) The expression of MMP2 was determined by qPCR in CAKI-1 and 786-O cells treated with miR-34c-5p mimic. (f) The expression of MMP2 was tested by Western blot analysis in CAKI-1 and 786-O cells treated with MMP2-AS1 siRNA, or co-treated with miR-34c-5p inhibitor. ∗∗P < 0.01.