Table 2.

Renal and cardiac mRNA expression of SARS-CoV-2 host factors and genes involved in RAAS

	Sham + ND + PBO (n = 6)	5/6Nx + ND + PBO (n = 6)	5/6Nx + HSD + PBO (n = 5–6)	5/6Nx + HSD + TELM (n = 6)	5/6Nx + HSD + LINA (n = 6)	5/6Nx + HSD + EMPA (n = 6)
mRNA expression (kidney)
Ace2	1.02 ± 0.09	1.19 ± 0.30	1.01 ± 0.33	1.14 ± 0.14	1.47 ± 0.47	1.33 ± 0.33
Tmprss2	1.03 ± 0.12	0.50 ± 0.09	0.86 ± 0.09	2.11 ± 0.41b	0.97 ± 0.08	4.23 ± 2.92b
Ren	1.06 ± 0.18	0.06 ± 0.02ab	0.06 ± 0.04a	1.56 ± 0.56b	0.12 ± 0.03a	2.52 ± 1.17b
Agtr2	1.03 ± 0.13	0.78 ± 0.21	1.08 ± 0.19	1.02 ± 0.13	0.84 ± 0.16	3.09 ± 1.41
Agt	1.10 ± 0.24	1.13 ± 0.28	1.02 ± 0.24	1.63 ± 0.29	1.57 ± 0.17	4.32 ± 1.67
mRNA expression (heart)
Ace2	1.04 ± 0.19	0.94 ± 0.06	1.17 ± 0.08	1.08 ± 0.08	0.77 ± 0.06	1.03 ± 0.08
Tmprss2	n.d	n.d	n.d	n.d	n.d	n.d
Ren	n.d	n.d	n.d	n.d	n.d	n.d
Agtr2	1.01 ± 0.06	1.06 ± 0.07	1.13 ± 0.09	0.98 ± 0.06	0.91 ± 0.09	0.97 ± 0.5
Agt	1.04 ± 0.12	1.79 ± 0.23	0.95 ± 0.04	1.19 ± 0.20	0.77 ± 0.09	0.92 ± 0.07
	Sham + ND + PBO (n = 12–14)	5/6Nx + ND + PBO (n = 7–12)	5/6Nx + HSD + PBO (n = 15–20)	5/6Nx + HSD + TELM (n = 7–11)	5/6Nx + HSD + LINA (n = 10–13)	5/6Nx + HSD + EMPA (n = 6–9)
protein expression
ACE2 (kidney)	29.89	27.10	13.96	14.14	33.25	17.07
	(20.82–36.55)	(19.39–30.73)	(12.36–19.14)a	(9.35–19.34)a	(18.55–39.25)b	(12.59–19.96)
ACE2 (heart)	27.77	33.11	29.12	27.42	24.39	33.21
	(25.58–32.96)	(30.74–36.34)	(23.97–32.42)	(24.57–31.01)	(22.32–29.63)	(26.57–35.92)
TMPRSS2 (kidney)	13.44	13.88	12.77	12.35	12.21	11.60
	(11.64–17.31)	(11.89–15.73)	(8.53–14.21)	(10.79–18.26)	(9.72–13.90)	(10.62–13.29)
TMPRSS2 (heart)	11.90	29.57	25.23	10.40	7.85	12.00
	(10.63–15.00)	(25.12–33.16)a	(16.18–30.46)a	(8.60–18.57)b	(6.03–13.39)b	(8.59–16.27)b

Normally distributed data were given as mean ± SEM. Non-normally distributed data were given as median × 10⁶ (25th–75th percentile × 10⁶). ^ap < 0.05 vs. Sham + ND + PBO, ^bp < 0.05 vs. 5/6Nx + HSD + PBO