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. 2022 Feb 21;298(4):101761. doi: 10.1016/j.jbc.2022.101761

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Kinetic assays of SbCPR2s with different electron acceptors.A, kinetic assay in dependence on cytochrome c concentration with constant NADPH concentration, B, kinetic assay for dependence on NADPH concentration with constant cytochrome c concentration. C, kinetic assay with C3′H in dependence on p-coumaroyl shikimic acid. D, kinetic assay with C4H in dependence on 2-naphthoic acid as an alternative substrate. Velocities are normalized by enzyme concentrations. C3′H, p-coumaroyl shikimate/quinate 3′-hydroxylase; C4H, cinnamate 4-hydroxylase.