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Interaction between SbC4H1 and truncated SbCPR2b.A, continuous absorbance spectrum for SbC4H1 when reduced by CPR. Arrows showed the direction of spectrum change through time. The experiment was conducted with both enzymes’ membrane-anchoring peptide being truncated. Low ionic strength was provided with CO-saturated 20 mM Hepes buffer. B, the percentage of reduced SbC4H1 is plotted versus time, and the total amount of SbC4H1 was 1 μM. CPR, cytochrome P450 reductase.
