Table 1.
Enzymatic parameters showing the comparison of SbCPR2s
| CPR type | Cytochrome c reduction |
C3′H reduction |
C4H reduction |
|||||
|---|---|---|---|---|---|---|---|---|
| Substrate | ||||||||
| Cytochrome c |
NADPH |
p-Coumaroyl shikimic acid |
2-Naphthoic acid |
|||||
| KM (μM) | Vmax (min−1)a | KM (μM) | Vmax (min−1)a | KM (μM) | Vmax (min−1)a | KM (μM) | Vmax (min−1)a | |
| SbCPR2a | 26.6 ± 3.3 | 2438.2 ± 99.7 | 5.3 ± 0.5 | 940.9 ± 22.8 | 69.6 ± 7.0 | 163.9 ± 69.6 | 1.6 ± 0.3 | 6.0 ± 0.3 |
| SbCPR2b | 48.9 ± 7.8 | 2432.8 ± 159.7 | 8.9 ± 1.0 | 950.7 ± 29.5 | 47.1 ± 6.6 | 369.8 ± 22.1 | 2.3 ± 0.4 | 30.1 ± 1.7 |
| SbCPR2c | 48.6 ± 11.5 | 3460.7 ± 337.4 | 14.9 ± 3.1 | 992.0 ± 14.9 | 186.8 ± 48.8 | 387.0 ± 66.5 | 0.3 ± 0.1 | 17.4 ± 0.9 |
a
Velocity was normalized by enzyme concentration.