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Parameters of CPR-dependent reduction of SbC4H in proteoliposomes
| System | Substrate | NADPH consumption, min−−1 | Oxygen consumption, min−1 | Product formation, min−1 | Coupling, % | H2O production, % |
|---|---|---|---|---|---|---|
| SbCPR–SbC4H1 | None | 28.5 ± 4.2 | 27.0 ± 0.3 | N/A | N/A | 5.5 ± 15.1 |
| 2-Naphthoic acid | 108.2 ± 7.0 | 88.1 ± 8.2 | 26.3 ± 4.4 | 25.6 ± 1.5 | 23.5 ± 9.0 | |
| Rat CPR–SbC4H1 | None | 12.5 ± 0.2 | 11.6 ± 0.3 | N/A | N/A | 8.0 ± 1.5 |
| 2-Naphthoic acid | 12.3 ± 1.3 | 10.2 ± 0.2 | 0.8 ± 0.2 | 6.2 ± 0.7 | 20.6 ± 15.0 |
Abbreviation: NA, not applicable.
