Table 3.
Summary of acyl-lysine modifications identified in S. aciditrophicus
| Modification | Proteins identified | Sites identified | Cyclized immonium ion m/z | Peptides with cyclized immonium ion (%) |
|---|---|---|---|---|
| Benzoylation | 1 | 2 | 188.107 | 100% |
| 3-Hydroxypimelylation | 16 | 20 | 224.129, 242.139 | 75%, 55% |
| Glutarylation | 4 | 6 | 198.112 | 100% |
| Crotonylation | 2 | 2 | 152.107 | 100% |
| 3-Hydroxybutyrylation | 4 | 6 | 170.118 | 83% |
| Acetylation | 48 | 104 | 126.091 | 94% |
| Butyrylation | 3 | 3 | 154.123 | 33% |
For each type of modification, the number of unique proteins bearing the modification and the total number of sites observed to be modified are presented. For the modified sequences, the percentages of MS/MS spectra that contained the diagnostic ions are stated.