Extended Data Fig. 6. Characterization of the TAMRA-D-R₈ enantiomeric peptide and its cellular uptake.

a, TAMRA-D-R₈ structure. b, RP-HPLC [Agilent SB-C18 column, H₂O (0.1% TFA)/CH₃CN (0.1% TFA) 95:5→5:95 (0→12 min)], R_t: 5.96 min. c, ESI-MS of TAMRA-D-R₈. d, Fluorescence images of HeLa cells obtained after incubation with 1 µM of TAMRA-D-R₈ and the indicated concentrations of B₁₂Br₁₂²⁻ for 30 min and washing; bright-field images in insets; all scale bars are 50 µm; the second panel (1 µM) is also shown in Fig. 3 in main text. Representative images of two biological replicates. Note that the clusters did not affect uptake of the non-conjugated TAMRA probe (see Supplementary Note 3).

Source data