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. 2022 Feb 1;603(7902):706–714. doi: 10.1038/s41586-022-04474-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — a. Graphical representation of Omicron spike mutations present in expression plasmid used to generate lentiviral pseudotyped virus (PV). Mutations coloured according to location in spike; bold mutations are novel to this lineage and have not been identified in previous VOCs. b. western blots of pseudotyped virus (PV) virions from 293T producer cells following transfection with plasmids expressing lentiviral vectors and SARS-CoV-2 S plasmids. (WT- Wuhan-1 with D614G), probed with antibodies for HIV-1 p24 and SARS-Cov-2 S2 (top) and S1 (bottom). c, d. quantification of western blots showing c. ratio of spike:p24 in virions, d. ratio of S2:total spike. Mean ratio +/- sd is shown for 3 biological replicates e. Western blot of cell lysates used to produce virions with f. quantification of S2 to total spike ratio. Mean ratio +/- sd is shown for 3 biological replicates g. brightfield images of lower airway organoids h. brightfield images of of cholangiocyte organoids (Scale bars 200 μm). i. infection schematic for entry assays in organoids created with BioRender.com.