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. 2022 Feb 1;603(7902):706–714. doi: 10.1038/s41586-022-04474-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a. Rendering of the contacts formed between the Omicron RBD (light blue) and human ACE2 (dark green) using PDB 7TN0 described in¹⁵ b. Zoomed-in view showing select amino acid side chains participating in remodelling of interactions between the Omicron RBD and ACE2. c–e. Biolayer interferometry binding analysis of human ACE2 to immobilized SARS-CoV-2 Wuhan-Hu-1 c. Delta d. or Omicron e. RBDs. Black lines correspond to a global fit of the data using a 1:1 binding model. f. Flow cytometry analysis of ACE2 binding to SARS-CoV-2 Wuhan, B.1.1.7 (Alpha), B.1.617.2 (Delta) or Omicron (B.1.1.529) spike proteins transiently expressed in ExpiCHO cells. g. Expression level of each spike as measured via binding by S2-subunit targeting S2P6 mAb. Grey histograms represent background florescence of cells stained with the secondary antibody only. h. Normalized ACE2 binding results based on spike protein expression levels. Values on the Y axis indicate the ratio between the mean fluorescence intensity (MFI) of positive cells stained for ACE2 binding and the MFI of the S2P6 positive cells. Data points in h. are mean of technical triplicates and data shown are representative two independent experiments.

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