Figure 9.

Immunostaining for GFP is below the threshold for detection in coronal brain sections derived from either naive wild type mice or CB₂^EGFP mice treated with either paclitaxel or its cremophor-based vehicle. Paclitaxel (4 mg/kg i.p. administered on day 0, 2, 4, and 6) or vehicle was administered on 4 alternate days, and mice were perfused and tissue dissected during the maintenance phase of CIPN (day 16). Photomicrograph of coronal section of the forebrain in (A) untreated wild type mouse, (B) cremophor vehicle–treated CB₂^EGFP mouse, and (C) paclitaxel-treated CB₂^EGFP mouse was labeled with DAPI (blue) and anti-GFP revealed by AlexaFluor 594 (red). Similar results were observed at all brain levels surveyed throughout the CNS. Scale bar in image = 100 µm. CIPN, chemotherapy-induced peripheral neuropathy; CNS, central nervous system; EGFP, enhanced green fluorescent protein; GPF, green fluorescent protein.