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. 2020 Nov 27;42(4):1005–1020. doi: 10.1007/s10571-020-00991-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Basic characteristics of glioblastoma-derived models in vitro. The study included three different ex vivo GB models: adherent culture supplemented with 10% FBS (10% adh); serum-free adherent culture (0% adh) and serum-free spheroid culture (0% sph). G108-, G114- and G116- derived cells were able to grow in all culture conditions, while G113 did not create stable spheroids. The status of neoplastic cell presence in culture was verified with the use of AAAs: IL13Rα2, EphA2 and Fra-1. All GB-derived culture models manifested expression of examined markers (a). The representative microphotographs of immunofluorescence results revealing AAAs presence in GB-derived cells in vitro (b)