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. 2022 Jan 26;18(3):1113–1126. doi: 10.1007/s12015-021-10261-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Characterization of EVs isolated from adipose stromal/stem cells (ASCs) culture supernatant. EVs from the ASC supernatant were isolated via differential (ultra)-centrifugation. (A) One representative micrograph of EVs isolated from ASCs taken by scanning helium-ion microscopy. (B) One representative graph of ASC-EV size evaluation using NanoSight nanoparticle tracking (NTA) analysis. ASC-EVs isolated from cell culture supernatant show a mean size of 157 nm. (C) MACSPlex analysis of the ASC-EV surface markers