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. 2022 Jan 8;30(1):43–57. doi: 10.1007/s10577-021-09678-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — FRAP analyses of CENP-H-mScarlet during interphase and mitosis. (A) Immunoblot analysis of cells in which endogenous CENP-C was replaced with GFP-fused CENP-C^WT and endogenous CENP-H was replaced with CENP-H-mScarlet (right). In the cell line in the left lane, only CENP-C was replaced with CENP-C-GFP. The indicated antibodies were used for immunoblot analysis. An asterisk indicates non-specific bands. (B) Localization of CENP-C-GFP (green) and CENP-H-mScarlet (red). DNA was stained with DAPI (blue). (C) FRAP analysis of cells expressing CENP-H-mScarlet during interphase and mitosis. Left panels are images before bleaching. The boxed area was bleached, and signal recovery is shown at the indicated time points. The black scale bar indicates 10 μm. The white scale bar indicates 2.5 μm for magnified panels. (D) Solid lines display the means of quantitative FRAP measurements for CENP-H-mScarlet from at least three cells for each line. The colored area between the two dashed lines indicates the standard deviation for each time point