Table 1.
Single-site changes to structural proteins affect the way WT and vaccine-specific mAbs bind YFV.
| MAB 117 | MAB 411 | LIVER MRP | BRAIN MRP | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amino acids | Virus backbone | Virus backbone | Asibi backbone | 17D backbone | Asibi backbone | 17D backbone | ||||||||
| RESIDUES | Asibi | 17D | Asibi | 17D | Asibi | 17D | Log10 change | Significance (p-value) | Log10 change | Significance (p-value) | Log10 change | Significance (p-value) | Log10 change | Significance (p-value) |
| FULL LENGTH | + | − | − | + | 1.8 | <0.0001 | 0.4 | 0.25 | 0.4 | 0.98 | 1.9 | 0.0023 | ||
| PRME | − | + | + | − | 0.1 | 0.99 | 2.1 | <0.0001 | 1.4 | <0.0001 | 0.3 | 0.21 | ||
| EDIII | + | − | + | − | 0.4 | 0.23 | 0.04 | 0.98 | 1.1 | <0.0001 | 0.08 | 0.87 | ||
| M-36 | L | F | − | + | − | − | ||||||||
| E-52 | G | R | + | + | − | + | 0.2 | 0.35 | 1.1 | <0.0001 | 0.4 | 0.013 | 1.1 | <0.0001 |
| E-170 | A | V | − | + | − | + | 0.09 | >0.9999 | 1.4 | <0.0001 | 0.8 | <0.0001 | 0.4 | 0.04 |
| E-173 | T | I | − | + | − | + | 0.2 | 0.34 | 0.9 | <0.0001 | 0.09 | 0.81 | 0.2 | 0.35 |
| E-200 | K | T | + | + | − | + | 1.6 | <0.0001 | 0.5 | 0.0021 | 1.6 | <0.0001 | 0.5 | 0.0021 |
| E-299 | M | I | + | + | +/− | − | 0.6 | 0.0003 | 1.1 | <0.0001 | 1.5 | <0.0001 | 0.7 | <0.0001 |
| E-305 | S | F | + | − | + | − | 1.9 | <0.0001 | 0.1 | 0.62 | 3.3 | <0.0001 | 0.4 | 0.047 |
| E-325 | P | S | + | − | + | − | 0.7 | 0.0001 | 0.2 | 0.53 | 1.1 | <0.0001 | 2.2 | <0.0001 |
| E-380 | T | R | + | − | + | − | 1.4 | <0.0001 | 0.2 | 0.25 | 2 | <0.0001 | 3.3 | <0.0001 |
| E-407 | A | V | − | +/− | + | + | 1.5 | <0.0001 | 2.5 | <0.0001 | 0.5 | 0.0083 | 0.2 | 0.44 |
Amino acid changes that differentiate Asibi and 17D are listed. The ability of WT (mAb 117) and vaccine (mAb 411) specific mAb was tested using structural chimeras of both Asibi and 17D viruses and immunofluorescent microscopy. Positive (+), negative (−), and equivocal (+/−) fluorescence.