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. 2022 Mar 23;5:255. doi: 10.1038/s42003-022-03218-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Density function for several CYCIF and ME-VAE feature pairs. A two-sided ANOVA was performed for features and intensities between populations in order to compute the F statistic and p value (PR(>F)). Subsequently, the mean Tukey pairwise p value across ligands and mean effect size are shown for each feature. ME-VAE features used for comparison were the features with the largest correlation to the respective CYCIF marker. This analysis utilized all 73,134 cell images from the MCF10A dataset.