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. 2022 Mar 23;5:256. doi: 10.1038/s42003-022-03191-5

Fig. 5. OZITX sensitivity of Gαi subfamily carboxy-tail Asn347/348 mutants.

Fig. 5

a Gαi3-WT activation, n = 11. b GαoA-WT activation, n = 4. c Gαz-WT activation, n = 5. d Gαi3-N347A activation, n = 4. e GαoA-N347A activation, n = 4. f Gαz-N347A activation, n = 6. g Gαi3-C351 activation, n = 4. h GαoA-C351 activation, n = 4. The G-protein activation assay was performed on WT, Asn347Ala/Asn348Ala (putative OZITX site) and Cys351Ile (PTX-insensitive) mutants. Cells were transfected with the D2R, the particular Gα mutant, the G-protein activation sensors and either a pcDNA3.1+ control (black open circles), OZITX-S1 cDNA (blue open circles) or PTX-S1 cDNA (red open circles). Cells were then stimulated with increasing concentrations of quinpirole before BRET detection. Data represent the mean drug-induced increase in BRET ratio from vehicle ± SEM. Individual replicates are shown as small circles.