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. 2022 Mar 23;5:253. doi: 10.1038/s42003-022-03206-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Five identical camera arrays were mounted on an air supported table. The associated workstations to run the Motif software were arranged in the two server racks underneath. b 3D schematic drawing of a single imaging unit (Kastl–Highres). The six cameras were mounted on a plate that is connected to the rig frame by three spring-loaded screws, and can be moved along the vertical axis. This allows for changing the focal plane of all six cameras at once. One of the imaging unit’s side panels is omitted from this view. c Technical drawing (front and side view) of an imaging unit annotated with dimensions in millimetres. d Using five identical units, 480 wells can be recorded simultaneously. Zooming in to the (e) camera, (f) well, (g) and worm level shows that this system achieves enough resolution to precisely track the nematodes. Each square well measures 8 × 8 mm.