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. 2022 Mar 22;12:4917. doi: 10.1038/s41598-022-08908-4

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Eye pigmentation phenotypes for the CRISPR/Cas9 Lygus hesperus mutant strains. (a) Egg (E) and nymphal instars (1st, 2nd, 3rd, 4th, and 5th) corresponding to representative wild-type, Card, and Cinn insects. Insets show 2.5 × magnification of the 3rd instar eye, which underscores gradual reversion to wild-type coloration in the Card line. (b) Ventral and lateral views of female and male eyes at 1-day and 7-days post-emergence.