Fig. 3.

Development and optimization of a robust extraction methodology for phenols. (A) Ultrasonication extraction in 80% methanol showed better recovery of phenolics and other metabolites (B) MTBE-based phase separation showed better enrichment for phenolics. (C) Venn diagram displaying the common and unique metabolites under 80% methanol and MTBE extracts (D) Variable Importance in Projection (VIP) score pattern analysis revealed that MTBE recovered phenolics from 80% methanol extracts which primarily showed sugars and organic acids