Table 1.
Phenolics detected in Arabidopsis thaliana rosette by GC-MS of MTBE extracts. The formula, molar mass, fragments obtained by MSTFA derivatives, identifier ions and their respective LOD and LOQ are listed. A representative GC-MS spectrum of phenolics is provided in supplementary Fig. S2. TMS represent trimethylsilyl, MO represents methyl oxime and “nd” represents Not determined
| Elution time | Phenolics | Formula | Molar mass (g/mol) | Derivatives | Sialylation Formula | MW | Identifier ions (m/z) | LOD (ng/ml) | LOQ (ng/ml) |
|---|---|---|---|---|---|---|---|---|---|
| 13.19 | Benzoic acid | C7H6O2 | 122.12 | 1TMS | C10H14O2Si | 194 | 179, 194, 135, 105 | nd | nd |
| 18.04 | Hydroxybenzoic acid | C7H6O3 | 138.12 | 2TMS | C13H22O3Si2 | 282 | 282, 267, 223, 193, 207 | 27.81 | 84.27 |
| 19.55 | Vanillic acid | C8H8O4 | 168.14 | 2TMS | C14H24O4Si2 | 312 | 312, 297, 282, 267, 253 | 37.31 | 113.05 |
| 19.85 | Coumaric acid | C9H8O3 | 164.04 | 2TMS | C15H24O3Si2 | 308 | 308, 293, 249, 219, 179 | 19.03 | 57.67 |
| 19.96 | Vanillin | C8H8O3 | 152.15 | MO TMS | C12H19NO3Si | 253 | 223, 253, 191, 206 | 43.55 | 131.96 |
| 20.19 | Protocatechuic acid | C7H6O4 | 154.12 | 3TMS | C16H30O4Si3 | 370 | 193, 370, 355, 311, 281 | nd | nd |
| 22.06 | Gallic acid | C7H6O5 | 170.12 | 4TMS | C19H38O5Si4 | 458 | 458, 281, 399, 311, 355 | 22.16 | 67.14 |
| 23.76 | Cinnamic acid | C9H8O2 | 148.15 | 1TMS | C12H16O2Si | 220 | 220, 205, 161, 145, 131 | 27.74 | 84.06 |
| 24.30 | Ferulic acid | C10H10O4 | 194.18 | 2TMS | C16H26O4Si2 | 338 | 338, 323, 308, 249, 219 | 33.38 | 50.57 |
| 24.65 | Sinapinic acid | C11H12O5 | 224.21 | 2TMS | C17H28O5Si2 | 368 | 280, 310, 295, 249 | nd | nd |
| 25.45 | Caffeic acid | C9H8O4 | 180.16 | 3TMS | C18H32O4Si3 | 396 | 396, 381, 219, 191 | 20.84 | 63.12 |