Fig. 6.

The beneficial effects of overexpression of NRF2 pathway to protect H9c2 cardiomyocytes from HH/R-induced ferroptosis were reversed by erastin. A Cell viability was detected by CCK-8. B ROS production was measured by adding the fluorescent probes to the cells during 20 min at 37 ℃. Scale bars: 100 μm. C Fe²⁺ content, MDA, and SOD levels in H9c2 cells were detected. D The protein levels of NRF2, FPN1, ACSL4, and GPX4 in myocardial tissue were detected by western blot. E The mRNA levels of NRF2, ACSL4, and GPX4 in myocardial tissue were detected by qRT-PCR. ^◆P < 0.05 vs. DIR group. ^▲P < 0.05 vs. DIR + SFN group. SFN sulforaphane, Era erastin