FIGURE 2.

a, b) Representative overview micrographs of cryobiopsies from asthmatic bronchi. c–e) Examples of robust immunohistochemical identification of airway mucosal immune cells (c, d: green, MPO⁺ neutrophils; brown, CD68⁺ macrophages; e: green MCt and brown MCtc mast cells). f, h) Detection of the positive control gene UBC mRNA in situ hybridisation (ISH). g) Negative ISH control with probe against the soil bacteria gene DapB. Ep: epithelium; sm: smooth muscle. Scale bars: a, 250 µm; b, 350 µm; c, 70 µm; d–e, 25 µm; f–g, 120 µm; h, 50 µm.