Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 17;21:15347354221086900. doi: 10.1177/15347354221086900

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 2. — Hesperetin inhibits the migration and invasion of MDA-MB-231 cells induced by TGF-β1. (A) Wound healing assay was applied to observe the inhibitory effect of hesperetin on the migration of MDA-MB-231 cells induced by TGF-β1. Cells were treated with TGF-β1 positive control or co-incubation of TGF-β1 and (25, 50, and 100 μM) hesperetin. Photos were taken with Keyence fluorescent microscope at 10× magnification at 0, 24, 48, and 72 hour after treatment. Scale bar = 100 μm. (B) The treatment of hesperetin inhibits dose-dependently migration induced by TGF-β1 in MDA-MB-231 cells. Cell migration rate was analyzed using NIH ImageJ software. Each result represents mean ± SD of 6 independent experiments. *P < 0.05 versus control group; #P < 0.05 versus TGF-β1 group; ## P < 0.01 versus TGF-β1 group. (C) Transwell invasion assay was used to evaluate the inhibitory effect of hesperetin on the invasion of MDA-MB-231 cells induced by TGF-β1. a: Control; b: TGF-β1; c: TGF-β1 + hesperetin 25 μM; d: TGF-β1 + hesperetin 50 μM; e: TGF-β1 + hesperetin 100 μM. Photos were taken with Keyence microscope at 10 × magnification at 48 hour after treatment. (D) The statistical figure results show that the TGF-β1-mediated invasion was significantly decreased in a dose-dependent manner in MDA-MB-231 cells after treatment with hesperetin. The number of invading cells was counted using NIH ImageJ software, and statistical analysis of results was analyzed with Graphpad Prism 8.4 software. Data are expressed as the mean ± SD, n = 6. **P < 0.01 versus control group; ##P < 0.01 versus TGF-β1 group.