Figure 7. NcDase modulates the expression of SCD1 and MUFAs levels in the liver by IgA-bound bacteria.

(A-D) Eight-week-old male GF C57BL/6 mice (n = 5) were orally administered IgA-bound microbiota from HFD-fed NcDase^−/− (GFNcDase) or WT (GFWT) mice and housed for 3–5 weeks.

(A) mRNA levels of the SCD1 and ELOVL5 in the liver after microbiota transplantation.

(B) Hepatic MUFAs in in total hepatic lipid, TG, CEs and PLs.

(C) Relative abundance of indicated bacteria in GF mice colonized with IgA-bound microbiota.

(D) Correlation between MUFAs and Ruminococcaceae abundance in GF mice colonized with IgA-bound microbiota from WT mice. Mean ± SEM; n = 5, *P < 0.05, **P < 0.01, ***P < 0.001.

(E-G) GF mice with colonization of PBS, Ruminococcaceae (Rumino), Desulfovibrio. desulfuricans (DSV) or Both housed for 3 weeks. Mean ± SEM; n = 4, *p < 0.05, **p < 0.01,

(E) SCD1 mRNA expression in the liver and in the ileum.

(F) SCD1 mRNA expression in the liver.

(G) Immunoblots of SCD1 in the liver.

(H) SCD1 mRNA expression in primary hepatocytes incubated for 12 hours with media or bacterial supernatant (SP).

Abbreviation: GAPDH, glyceraldehyde 3-phosphate dehydrogenase.