Appendix Table 1.
Membrane viscosity obtained using different experimental methods and simulations
| Method | Membrane composition | Viscosity (nPa·s·m) | Temperature (K) | System |
|---|---|---|---|---|
| Falling ball viscometry (27) | SOPC | 3 | 298 | GUVs |
| Coarse-grained simulations (28) | SOPC | 3 | 323 | bilayer |
| Electrodeformation (this study) | SOPC | 9.73 ± 5.80 | 298 | GUVs |
| Probe diffusion (61) | SOPC | 13.2 a | 293 | GUVs |
| Optical dynamometry (26) | DMPC | 5 ± 2 | 298 | GUVs |
| Neutron spin echo (20) | DMPC | 75 | 298 | SUVs |
| Coarse-grained simulations (28) | DMPC | 5 | 323 | bilayer |
| Fluorescence spectroscopy (79) | DMPC | 0.36 a | 298 | SUVs |
| Tether pulling (11) | EPC | 2.7–88 | 295–298 | GUVs |
| Shear surface rheology (10) | POPC | 300,000 | NA | monolayer |
| Electrodeformation (this study) | POPC | 9.32 ± 5.95 | 298 | GUVs |
| Shear surface rheology (10) | POPC:Chol (7:3) | 10,000 | NA | monolayer |
| Shear surface rheology (10) | DPPC | 900,000 | NA | monolayer |
| Optical tweezers (80) | DOPC | <0.6 | 293 | bilayer |
| Fluorescence spectroscopy (79) | DOPC | 0.20 a | 298 | SUVs |
| Fluorescence quantum yield (23) | DOPC | 0.84 a | 298 | LUVs |
| Fluorescence lifetime of dye (24) | DOPC | 0.15 a | 298 | LUVs |
| All-atom simulations (7) | DOPC | 0.197 ± 0.0069 | 297 | bilayer |
| Coarse-grained simulations (28) | DOPC | 0.17 | 323 | bilayer |
| Membrane-anchored particles (16) | DOPC | 15.3 ± 3.4 | 297 | GUVs |
| Electrodeformation (this study) | DOPC | 4.11 ± 2.63 | 298 | GUVs |
| Probe diffusion (15) | DOPC | 0.59 ± 0.2 | 298 | GUVs |
| Neutron spin echo (9) | DOPC | 16.7 ± 1.1 | 298 | SUVs |
| Electrodeformation (this study) | OMPC | 7.73 ± 3.09 | 298 | GUVs |
| Membrane-anchored particles (16) | 13:0 PC | 14.7 ± 6.9 | 297 | GUVs |
| Neutron spin echo (9) | DOPC:Chol (8:2) | 31.9 ± 3.5 | 298 | SUVs |
| Electrodeformation (this study) | DOPC:Chol (1:1) | 7.00 ± 4.77 | 298 | GUVs |
| Electrodeformation (this study) | DPPC:Chol (1:1) | 56.4 ± 4.63 | 298 | GUVs |
| Optical tweezers (80) | DOPC:DPPC (2:1), Lo | 2.1 | 293 | bilayer |
| Shear-driven flow (8) | DOPC:DPPC (85:15), Ld | 1.9 ± 11.9 | 296 | GUVs |
| Shear-driven flow (8) | DiPhyPC:Chol:DPPC (5:40:55), Lo | 15.7 ± 9.9 | 296 | GUVs |
| Domain fluctuations (18) | DiPhyPC:Chol:DPPC (25:55:20) | 4 ± 1 | 293 | GUVs |
| Diffusion of domains (12) | DPPC:DOPC:Chol (3.5:3.5:3), Lo | 10–500 | 295 | GUVs |
| Diffusion of domains (13) | DiPhyPC:DPPC (1:1) | 2.2 ± 0.1 | 296.5 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (3:6:1), Lo domain | 4.1 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (4:5:1), Lo domain | 14 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (2:6:2), Lo domain | 9.6 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (4:4:2), Lo domain | 4.3 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (5:3:2), Lo domain | 54 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (6:2:2), Ld domain | 1200 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (2:5:3), Lo domain | 7.7 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (5:2:3), Ld domain | 85 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (6:1:3), Ld domain | 1200 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (3:3:4), Ld domain | 0.63 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (4:2:4), Ld domain | 65 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (5:1:4), Ld domain | 71 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (4:3:3), Ld domain | 98 | 293 | GUVs |
| Shear-driven flow (19) | DPPC:DOPC:Chol (3:5:2), Lo domain | 65 | 293 | GUVs |
| Electrodeformation (this study) | DPPC:DOPC:Chol (1:1:1) | 17.7 ± 3.06 | 298 | GUVs |
| Electrodeformation (this study) | DPPC:DOPC:Chol (1:1:2) | 15.4 ± 2.40 | 298 | GUVs |
| Falling ball viscosimetry (81) | PBd33-b-PEO20 | 1500 ± 120 | 298 | GUVs |
| Electrodeformation (this study) | PBd33-b-PEO20 | 2890 ± 670 | 298 | GUVs |
| Micropipette aspiration (82) | PEG-b-PA6ester1 | 7,900,000 ± 200,000 | 298 | GUVs |
| Micropipette aspiration (82) | PEG-b-PA444 | 4,000,000 ± 200,000 | 298 | GUVs |
| Atomic force microscopy (83) | PBd130-b-PEO66 | 5,000,000 | 298 | bilayer |
| Atomic force microscopy (83) | PBd130-b-PEO66 (cross-linked) | 20,000,000 | 298 | bilayer |
| Electrodeformation (this study) | PBd13-b-PEO11 | 14.4 ± 4.40 | 298 | GUVs |
| Electrodeformation (this study) | PBd22-b-PEO14 | 686 ± 51.0 | 298 | GUVs |
| Electrodeformation (this study) | PBd46-b-PEO24 | 20,600 ± 4700 | 298 | GUVs |
| Electrodeformation (this study) | PBd54-b-PEO29 | 46,700 ± 900 | 298 | GUVs |
| Electrodeformation (this study) | PBd120-b-PEO78 | 157,000 ± 54,500 | 298 | GUVs |
Ld and Lo refer to liquid-disordered and liquid-ordered phases, respectively. GUVs, LUVs, and SUVs refer to giant unilamellar vesicles, large unilamellar vesicles, and small unilamellar vesicles, respectively.