Figure 3:

Altering metabolite distributions at cleavage stages perturbs foxj1 expression at gastrula stages.

(A) In control gastrula stage 10.5 embryos, foxj1 expression (purple) was equivalent in length on the right (R) and left (L) sides of the embryo. Using a video camera attached to a stereomicroscope, the midpoint of the pigmented cells forming the blastopore, was marked (red asterisk), a line was drawn from that point towards the animal pole (red line) and the length of the foxj1 expression domain measured from that line with an eyepiece reticule.

(B) Examples of embryos in which the Lmet cocktail was injected into the R-D1 blastomere.

(C) Lengths of the right and left domains of foxj1 expression in each embryo, in reticule units, were compared by a two-tailed, paired Wilcoxon test (* = p < 0.005). The right and left domains of foxj1 expression in Lmet-R-D1 embryos were significantly different in length when compared by a two-tailed, paired Mann-Whitney test (# = p < 0.0005). Bars indicate SEM and samples sizes are in parentheses.