Figure 4:

Altered Lmet concentration in R-D1 perturbs dand5 expression in the GRP.

(A) Expression of dand5 in the GRP is typically larger, longer and/or darker on the right (R) side compared to the left (L) side (left panel), particularly in control embryos. In a minority of control cases, it was longer on the left side (left middle), equivalent in length on both sides (right middle) or not expressed at the stage examined (right panel). Tissue is oriented from a ventral view with posterior (Post) to the top and anterior (Ant) to the bottom.

(B) The frequencies of left-right dand5 expression patterns were dramatically different between control embryos and siblings in which Lmet was injected into R-D1. Samples sizes are in parentheses.